Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Suaeda japonica Makino Attenuates Lipopolysaccharide-Induced Neuro-Inflammatory Responses in BV-2 Microglia via NF-kappa B Signaling

Hyun Kang^1,3, Sushruta Koppula¹, Hoi- Ki Kim², Tae-Kyu Park¹

¹Department of Biotechnology , Research Institute for Biomedical & Health Science, College of Biomedical and Health Science, Konkuk University, Chungju, 380-701; ²Fanipin Korea Co, Ltd, Ochang 208-211; ³KuGen Healthcare Institute, Chungju, 380-150, Republic of Korea.

For correspondence:- Tae-Kyu Park Email: tkpark@kku.ac.kr Tel:+82438403870

Received: 18 April 2013 Accepted: 23 May 2013 Published: 12 June 2013

Citation: Kang H, Koppula S, Kim H, Park T. Suaeda japonica Makino Attenuates Lipopolysaccharide-Induced Neuro-Inflammatory Responses in BV-2 Microglia via NF-kappa B Signaling. Trop J Pharm Res 2013; 12(3):351-356 doi: 10.4314/tjpr.v12i3.12

© 2013 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To evaluate in vitro anti-oxidant and anti-neuro-inflammatory activities of Suaeda japonica extract (SJE) in lipopolysaccharide (LPS)-stimulated BV-2 microglial cells.

Methods: 1, 1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging assay was used to study the antioxidant effects. 3-(4, 5-dimethylthiazol-2-yl)-2, 5- diphenyl-tetrazolium bromide (MTT) assay was used to study cell viabilities. LPS-stimulated BV- microglial cells were used to study the gene expression and production of inflammatory mediators determined by Western blot analysis.

Results: SJE significantly inhibited the DPPH generated free radicals, and suppressed LPS-induced expression of inducible nitric oxide synthase (iNOS) and production of nitric oxide (NO) in a concentration-dependent manner. It decreased LPS-induced expression of some inflammatory mediators and pro-inflammatory cytokines (cyclooxygenase-2 and interleukin (IL)-6). This suppression of inflammatory mediators was nuclear factor kappa-B (NF-κB)-dependent.

Conclusion: Our findings imply that SJE may be a potential therapeutic agent in regulating microglia-mediated neuroinflammatory responses observed in several neurodegenerative diseases.

Keywords: Suaeda japonica, Antioxidant activity, Anti-inflammatory activity, Microglial Cells, iNOS, IL-6